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Contents 2019-4
MEDICAL SCIENCE

PREVENTIVE MEDICINE

1. Role for bacteriophages in the evolution of healthcare - associated infection pathogens  L.P. Zueva, B.I. Aslanov, A.A. Dolgiy L.V., Belova           4
2. Study of dentists burnout working in pediatric dentistry A.O. Karelin, P.B. Ionov                      10
3. Microbiota of aquatic ecosystems associated with melting glaciers of the Svalbard archipelago: biodiversity and pathogenic potential

A.E. Goncharov,I.Y. Kirtsideli, D.Yu. Vlasov, D.V. Azarov, E.A. Lebedeva, V.V. Kolodzieva, N.E. Goncharov, V.S. Vargasova, L.A. Kraeva, L.V. Belova, L.P. Zueva          16

4. Risk of body' s accelerated aging as a reducing factor of reproductive potential in women V.S. Gladkaya, O.Yu. Kilina, V.L . Gritsinskaya, O.V. Chudinova              22
5. Genetic diversity of Blastocystis spp. in St. Petersburg and the Chukotka autonomous area D.V. Azarov, A.E. Goncharov, S.G. Belov, I.A. Kulemin, V.R. Kononova, L.P. Zueva                    30
6. Сomparative analysis of health status of employees working in an open territory in t cold period of the year according to questionnaire results  E.M. Polyakova, A.V. Meltser   35

CLINICAL MEDICINE

1. Use of biomaterial derived from human umbilical cord for the regeneration of articular cartilage

L.I. Kalyuzhnaya, V.V. Khominets, S.V. Chebotaryov, O.N. Kharkevich, A.L. Kudyashev, V.E. Chernov, A.S. Grankin, D.A. Zemlyanoy, R.A. Fedorov, D.V. Tovpeko                 45

2. Intraoperative radiation therapy in breast cancer treatment (review)

E.M. Bit-Sava, A.V. Miller, A.O. Damenia, M.A. Monogarova, M.G. Kurbanova, Sh.B. Bakhovadinova, R.M. Akhmedov, M.S. Ravkina, V.M. Moiseenko              53

3. Ways to improve treatment results of pancreatoduodenal resection

M.Yu. Kabanov, M.D. Hanevich, K.V. Sementsov, D.Yu. Boyarinov              60

4. Mobile application for patients as a structural element of the new clinical and laboratory model of centralized monitoring in warfarin treated patients

 M.V. Khruslov, M.A. Karpenko, I.V. Ponomareva, L.V. Safronova, T.V. Vavilova                        67

5. Comparative characteristic of patients with non–ST elevation acute coronary syndromes of different age groups 

E.A. Lavrinova, D.N. Bryliakova, G.A. Kukharchik                   72

6. Study of the immunotropic effects of flavone glycoside embinin by flow cytometry

 N.V. Bychkova, A.A. Kalashnikova, A K. Whaley, V.G. Luzhanin, N.M. Kalinina, E.B. Shustov, S.V. Okovity  77

PREVENTIVE MEDICINE

ROLE OF BACTERIOPHAGES IN THE EVOLUTION OF HEALTHCARE ASSOCIATED INFECTION PATHOGENS
L.P. Zueva, B.I. Aslanov, A.A. Dolgiy, L.V. Belova
North-Western State Medical University named after I. I. Mechnikov, Russia, 191015, Saint-Petersburg, Kirochnaya Street, 41.
Abstract.
Introduction. One of the major challenges of modern medicine is antibiotic resistance of hospital acquired infection pathogens. Thus, the detailed study of the mechanisms of their evolution is of a high importance.
The aim of the study was to evaluate the role of temperate bacteriophages circulating in hospital environment on the biological features of hospital acquired infection bacterial pathogens.
Materials and methods. The study was conducted in a number of medical facilities (surgical, traumatological, urological, oncological and burn departments, neonatal intensive care units). Isolation of hospital acquired infection bacterial strains was carried out in accordance with generally accepted bacteriological methods. Isolation of phages from clinical samples was carried out according to generally accepted methods, but in authors modification. Statistical analysis was performed using Statistica and WinPepi software.
Results. A strong correlation was revealed between the incidence of hospital acquired infection caused by E. coli and the intensity of E. coli circulation with prophage gene c2418, weak positive relationship was observed between the incidence of urinary tract infections and isolation rate of Enterococcus spp. with phage genes gp2 and yopX, a strong correlation was noted between the incidence of S. aureus HAIs and the intensity of circulation of temperate phages of S. aureus in the hospitals, a strong positive relationship was revealed between the activity of the circulation of Pseudomonas phages in the hospitals and the dynamics of P. aeruginosa hospital acquired infections.
Conclusion. It was established a significant role of temperate bacteriophages in evolution of pathogenic capacity of hospital acquired infection bacterial pathogens. Temperate bacteriophages promote the acquisition of important biological properties by hospital strains:  increased virulence and ability to epidemic spread, multiple resistance to antibiotics, disinfectants and antiseptics.
Keywords: healthcare associated infection, bacteriophage, antibiotic resistance.

STUDY OF DENTISTS BURNOUT WORKING IN PEDIATRIC DENTISTRY
A.O. Karelin, P.B.Ionov
Pavlov First Saint Petersburg State Medical University, Russia, 197022, Saint-Petersburg, Leo Tolstoy Street, 6-8
Abstract
Introduction. During the last decade professional burnout syndrome has been increasingly observed in dentists. Pediatric dentists are considered to be the group of specialists mostly exposed to occupational stress with the proposed high risk of developing professional burnout.
The aim of the study wasto analyze the prevalence and nature of the professional burnout development in dentists working in state pediatric institutions of Saint-Petersburg.
Materials and methods. A survey was conducted among 71 dentists (5 male and 56 females with average age 51.8 ± 11.34 years) from five medical institutions of Saint-Petersburg providing dental care for children, using Maslach Burnout Inventory burnout questionnaire adapted by N.E. Vodopyanova.
Results. The majority of dentists working in pediatric state dentistry are exposed to professional burnout. High level of professional burnout was found in 7% of dentists according to all three scales; 93% of dentists had high level of emotional exhaustion and depersonalization with an average or low level of reduction of professional achievements. Significant differences were found between groups of orthodontists, dental therapists and dental surgeons. The level of emotional exhaustion and depersonalization was significantly higher in dental surgeons and in orthodontists in comparison with dental therapists; the differences between the groups by the level of reduction of personal achievements were not revealed.
Conclusion.  It was studied the characteristics of professional burnout of dentists working in state pediatric dental institutions. The majority of dentists have a high level of emotional exhaustion and depersonalization. At the same time, they have a moderate level of reduction of personal achievements.
Keywords: occupational stress, burnout syndrome, pediatric dentist, orthodontist, surgeon

MICROBIOTA OF AQUATIC ECOSYSTEMS ASSOCIATED WITH MELTING GLACIERS OF THE SVALBARD ARCHIPELAGO: BIODIVERSITY AND PATHOGENIC POTENTIAL
A.E. Goncharov 1,2, I.Y. Kirtsideli3, D.Y. Vlasov3,4, D.V. Azarov1, E.A. Lebedeva1, V.V. Kolodzhieva1,2, N.E. Goncharov1, V.S. Vargasova1, L.A. Kraeva5 , L.V. Belova1, L.P. Zueva1
1North-Western State Medical University named after I.I Mechnikov, Russia, 191015, Saint- Petersburg, Kirochnaya Street., 41
2Institute of experimental medicine, Russia, 197376, Saint-Petersburg, Academician Pavlov Street, 12
3Komarov Botanical Institute of the Russian Academy of Sciences (BIN RAS),Russia,197376, Saint-Petersburg, Professor Popov street, 2P
4Saint-Petersburg State University, Russia, 199034, St. Petersburg, Universitetskaya Naberezhnaya 7-9
5Saint-Petersburg Pasteur Institute, Russia 197101, Saint- Petersburg, Mira Street., 14
Abstract
Introduction. The melting of permafrost and ice due to global warming in the polar regions of the Earth can lead to the activation of ancient pathogens and the introduction of their genetic material into modern ecosystems. This process increases the risk of epidemic outbreaks in the polar regions of the planet. Microbiological studies in areas of active melting of Arctic glaciers are of a high necessity.
The aim of the study was to assess the taxonomic composition of bacteria found in aquatic ecosystems associated with actively melting glaciers in Svalbard.
Material and methods. It was used a membrane filtration method for the aquatic microbiome investigation in water samples from glacial meltwater streams and lakes at Nordensheld Land, West Spitsbergen. The taxonomic composition of the isolated bacteria was studied using MALDI-TOF and 16s rRNA gene sequencing.
Results. Opportunistic bacteria, attributed to the Enterococcus, Pseudomonas, Yersinia, Stenotrophomonas; Escherichia, Acinetobacter genera were isolated among psychroactive microboita. It was described a strain of Acinetobacter calcoaceticus having a significant pathogenic potential.
Conclusion. The study confirms the importance of microbiological monitoring of glacial ecosystems as an element of assessment and prediction of biological risks associated with global climate warming.
Key words: microbiota, global warming, Arctic, infections, glaciers

RISK OF BODY ' S ACCELERATED AGING AS A REDUCING FACTOR OF REPRODUCTIVE POTENTIAL IN WOMEN
V.S. Gladkaya1, O.Yu. Kilina1, V.L. Gritsinskaya2, O.V. Chudinova1

Abstract
Introduction. The preservation of reproductive health in women and the realization of their reproductive potential is largely determined by the body’s aging rate. Defining the risk of premature body aging based on somatometric and clinical indicators is a promising method of personalized prevention.
The aim of the study was to identify the correlation of somatometric and clinical indicators with biological age and the aging rate of women of three age periods.
Materials and methods. It was examined 320 women of the indigenous (Mongoloids – Khakass) and alien (European – Russian) population of the Republic of Khakassia aged 21 to 55 years. They were measured the length and body weight; chest circumference and buttocks; shoulders and pelvis diameter; by calculation was determined the body mass index, biological age and coefficient of the aging rate; ROC statistical analysis was used to identify signs affecting the body's aging rate with an assessment of the methods diagnostic effectiveness (sensitivity, specificity, accuracy, prognostic significance), and to identify the level of quantitative diagnostic criteria using the calculation of chance and odds ratio.
            Results. The body’s accelerated aging rates revealed in 77,8% of Europeans and 37,5% of Khakass (p=0,005). In Europeans, the following indicators are associated with the risk of body’s accelerated aging: body mass index ≥ 25 kg/m2 (odds ratio criterion–65,4); chest circumference ≥ 85,5 cm (odds ratio –38,1); the duration of the menstrual cycle ≥ 30 days or more (odds ratio –4,1); pelvic diameter ≥ 27,5 cm (odds ratio –4,2). For Khakass-women with the risk of body’s accelerated aging, the following values are associated: body mass index ≥ 24,7 kg/m2 (odds ratio –27,3); chest circumference ≥ 89,5 cm (odds ratio –41,7); pelvis diameter ≥ 26,5 cm (odds ratio –5,0).
Conclusions. The use of the obtained data allow to form the risk group for women with conditions affecting the reproductive system for the timely correction.
Key words: women, somatometric indicators, biological age, aging rate

GENETIC DIVERSITY OF BLASTOCYSTIS SPP. IN SAINT- PETERSBURG AND CHUKOTKA AUTONOMOUS AREA
D.V. Azarov1,2, A.E. Goncharov1,3, S.G. Belov1, I.A. Kulemin2, V.R. Kononova2, L.P. Zueva1
1North-Western State Medical University named after I.I Mechnikov, Russia 191015, Saint- Petersburg, Kirochnaya Street, 41.
2Saint- Petersburg City Center for Hygiene and Epidemiology, Russia, Saint-Petersburg, Malaya Sadovaya Street, 1.
3Institute of experimental medicine, Russia,197376, Saint-Petersburg,Academica Pavlova Street, 12
Abstract
Introduction. Blastocystis spp. widespread intestinal protozoa of humans and animals. Approaches to the preventive measures’ development require theoretical justification including the information on the genetic diversity of Blastocystis spp.
The aim of the study was to assess of the genetic diversity of Blastocystis spp. circulating in the territory of two geographically distant subjects of the Russian Federation.
Materials and methods. It was performed the genetic subtyping of 144 blastocyst isolates obtained from residents of Saint-Petersburg (117 samples) and the Chukotka Autonomous Area (27 samples) using two methods: PCR with STS primers and fragment sequencing of the small subunit ribosomal RNA gene (Barcoding).
Results. The distribution of isolates by subtype was as follows: St. Petersburg: ST1 - 33 (28.7%), ST2 - 33 (28.7%), ST3 -32 (27.8%), ST4 -1 (0.9 %), ST7 - 2 (1.7%), ST2 + ST3 - 1 (0.9%), it wasn’t determined a subtype of 13 (11.3%), nonspecific amplification was observed in 2 samples; Chukotka Autonomous Area: ST1 - 6 (22.2%), ST2 - 5 (18.5%), ST3 -7 (25.9%), ST4 -1 (3.7%), ST7 - 1 (3, 7%), it wasn’t determined a subtype - 7 (25.9%).
Conclusion. The obtained data confirm the presence of genetic heterogeneity of Blastocystis spp. circulating among the population of Russia. Moreover, the absence of significant differences in the structure of the subtype’s distribution, both between the two analyzed regions and worldwide, indicates the global nature of the epidemic process of Blastocystis spp. The results of blastocysts genetic subtyping should be considered while preventive measures implemented.
Key words: Blastocystis spp., parasitology, genotyping, genetic diversity, sequencing

COMPARATIVE ANALYSIS OF HEALTH STATUS OF EMPLOYEES WORKING IN AN OPEN TERRITORY IN T COLD PERIOD OF THE YEAR ACCORDING TO QUESTIONNAIRE RESULTS
E.M. Polyakova, A.V. Meltser
North-Western State Medical University named after I. I. Mechnikov, Russia, 191015, Saint-Petersburg, Kirochnaya Street, 41.
Abstract
Introduction. The main reasons for considering oil industry employees to be a group with a high risk of general and occupational diseases are: the impact of a complex of harmful factors in the work environment, the work process along with adverse climatic conditions.
The aim of the study was to performa comparative analysis of the questionnaire results of oil industry employees working under conditions of intense exposure to cold environment in order to analyze their self-rated health, to identify risk factors and events leading to its deterioration, and to analyze the contribution of production factors to health status.
Materials and methods. It wassurveyed 596 employees of an oil company of various professional groups in comparison with a control group consisting of administrative and managerial staff. The use of the developed questionnaire allow to assess the employees’ health status of various professional groups, and to determine the risk factors when working in the open area in the cold season.
Results. According to the questionnaire results, it was found that employees working in cold environment have the proven risk of the following pathologies development: musculoskeletal disorders, skin disorders, psychoemotional disorders. The majority of occupational groups mainly associate their illnesses with cold environment. Employees, who expose to severe cold while working, are more likely to suffer from respiratory infections. Significant risk factors for the diseases development during cold exposure are smoking and alcohol. The questionnaire results reveal the metabolic disorders (increased body weight and obesity) in the exposed group, which is considered as a cold adaptation factor. At the same time, a significant number of employees believe that special clothing does not offer an adequate level of cold protection. Humid clothes are a significant risk factor while working in the open area for the development of cold-related diseases.
Conclusion. According to the questionnaire resultsand risk factors for health disorders in the result of the adverse climate environment were proposed medical and preventive measures.
Key words: oil company employees, workers' health, working in the cold environment, risk factors.

CLINICAL MEDICINE

USE OF BIOMATERIAL DERIVED FROM HUMAN UMBILICAL CORD FOR THE REGENERATION OF ARTICULAR CARTILAGE
L.I. Kalyuzhnaya, V.V. Khominets, S.V. Chebotaryov,
 O.N. Kharkevich, A.L. Kudyashev, V.E. Chernov, A.S. Grankin, D.A. Zemlyanoy, R.A. Fedorov, D.V. Tovpeko.
Military Medical Academy named after S.M.Kirov, Russia, 194044, Saint- Petersburg, Akademika Lebedeva Street, 6.
Abstract
Introduction. Articular cartilage damage, usually occurring in people with an active lifestyle, is a major risk factor of osteoarthritis development- inflammatory and degenerative joint disease. Wharton’s jelly - a solid mucous tissue surrounding the umbilical cord blood vessels and consisting of extracellular matrix and mesenchymal stem cells. Extracellular matrix has a similar structure to the hyaline cartilage matrix, represented by the different types of collagens, glycoproteins, proteoglycans, each of the components is considered to be a therapeutic agent for regenerative medicine.
The aim of the study was to develop the preparation methods of hydrogels derived from human umbilical cord and experimentally prove its effectiveness in treatment of hyaline cartilage damage.
Materials and methods. The study included 15 chinchilla rabbits, which were artificially formed with the bone cartilage defect of the loaded zone of the femur medial condyle. The first group of animals (8 rabbits) was given three times 0.4 ml 0.9% sodium chloride solution into the operated joint, as in the second group (7 rabbits) 0.4 ml - umbilical cord biomaterial hydrogel. After 60 days all animals were performed MRI of knee joints with the measuring of the depth and diameter of the defect.
Results. The average defect diameter in the first and second groups was 3.25 and 3.06 mm, respectively; the average depth in the first and second groups was 2.69 and 2.63 mm, respectively. A statistically significant increase in the defect size was observed in the group in which the saline solution was used; and a significant decrease were found in the defect depth in the group treated with umbilical cord hydrogel (p<0.05). It was not revealed any complications at intraarticular injection of umbilical cord biomaterial hydrogel.
Conclusion. Wharton’s jelly cell-free matrix as an injection hydrogel and injected into the cavity of traumatized knee joints of rabbits by the 60th day after the injury resulted into a partial reduction in the depth and area of cartilage defects.
Key words: matrix, scaffold, decellularization, Wharton’s jelly, umbilical cord, magnetic resonance imaging, osteoarthritis.

INTRAOPERATIVE RADIATION THERAPY IN BREAST CANCER TREATMENT (REVIEW)
Е.M. Bit-Sava 1,2, A.V. Miller 1, A.O. Damenia 1, M.A. Monogarova 1,
M.G. Kurbanova 1, Sh.B. Bakhovadinovа1,  R.M. Akhmedov1, M.S. Ravkina1,
V.M. Moiseenko 1
1Saint-Petersburg clinical scientific and practical center for specialized types of medical care (oncological), Russia, 197758, Saint Petersburg, village Pesochny, Leningradskaya street, 68A
2 Saint Petersburg State Pediatric Medical University, Russia, 194100, Saint Petersburg, Litovskaya street 2
Abstract

Keywords Breast cancer, intraoperative radiation therapy, external beam radiation therapy, accelerated partial breast radiation

WAYS TO IMPROVE TREATMENT RESULTS OF PANCREATODUODENAL RESECTION
M.YU. Kabanov1,2, M.D. Hanevich3, K.V. Sementsov1,2, D.Yu. Boyarinov2
1North-Western State Medical University named after I. I. Mechnikov, Russia, 191015, Saint-Petersburg, Kirochnaya Street, 41.
2Hospital for War Veterans, Russia, 194000, Saint-Petersburg, Narodnaya Street, 21
3City Clinical Oncology Dispensary, Russia, Saint-Petersburg, 194000, Veteranov Avenue,56
Abstract
Introduction. In recent decades it was observed an increase in the incidence of malignant tumors of the biliopancreatoduodenal zone by 4 times, characterized by a high mortality rate. One of the major treatment methods of malignant tumors of the biliopancreatoduodenal zone is surgical. Therefore, the improvement of the immediate results of surgical treatment is a prerequisite for the successful treatment of this group of patients.
The aim of the study was to improve the immediate results of pancreatoduodenal resection in patients with various diseases of the organs of the biliopancreatoduodenal zone.
Materials and methods. It was analyzed the surgical treatment results of 196 patients, who underwent pancreatoduodenal resection for the pancreaticobiliary disease of various genesis during 2002 - 2018.
Results. The use of optimized diagnostic and treatment tactics reduced the number of postoperative complications from 41% to 29%, while the incidence of pancreatic fistula decreased from 35% to 22%, and postoperative mortality from 15% to 7%.
Conclusion. The use of optimized therapeutic and diagnostic tactics in patients with pancreaticobiliary disease allows improving the immediate results of surgical treatment.
Keywords: pancreatoduodenal resection, pancreatic fistula, pancreaticenteroanastomosis

MOBILE APPLICATION FOR PATIENTS AS A STRUCTURAL ELEMENT OF NEW CLINICAL AND LABORATORY MODEL OF CENTRALIZED MONITORING OF WARFARIN TREATED PATIENTS
M.V. Khruslov1, 2, M.A. Karpenko2, I.V. Ponomareva3,
L.V. Safronova4, T.V. Vavilova2
1Kursk regional clinical hospital, Russia, 30500, Kursk, Sumskaya Street, 45A.
2Almazov National Medical Research Centre, Russia, 197341, Saint Petersburg, Akkuratova Street, 2.
3Kursk State Medical University, Russia, 305041, Kursk, Karl Marx Street, 3.
4Kursk polyclinic № 7,Russia, 305047, Kursk, Zavodskaya Street, 25.
Abstract
Introduction. Telemedicine allows to halve the number of hospitalizations of chronic patients. This type of medical care is especially relevant for patients with a high risk of thromboembolic complications, treated by vitamin K antagonists (warfarin).
The aim of the study was to improve the existing clinical and laboratory model for centralized monitoring of warfarin treated patients by developing a mobile application for these patients.
Materials and methods. The mobile application was developed on the basis of“Centralized Monitoring System of INR” where due to the information technologies, telecommunication systems within primary healthcare patient and outpatient facilities, a unified laboratory center and medical staff integrate into a one common system.
Results. A mobile phone application was developed and implemented into the treatment process, allowing to involve a patient into active communication with the doctors by access to the the medical history, transmit the tests results, if necessary, to fill an application for unscheduled consultation. Within 5 months, 548 patients started using the mobile application (34.5% of those observed patients being a part of the clinical and laboratory model of centralized monitoring). The time spent in the therapeutic range is increased up to 75%.
Conclusion. The implementation of a mobile application allows to improve patient adherence to treatment, the effectiveness and safety of warfarin therapy.
Key words: anticoagulant therapy, telemedicine, warfarin intake monitoring system.

COMPARATIVE CHARACTERISTIC OF PATIENTS WITH NON–ST-ELEVATION ACUTE CORONARY SYNDROMES OF DIFFERENT AGE GROUPS
E.A. Lavrinova 1, D.N. Bryliakova 2, G.A. Kukharchik 1, 2
1North-Western State Medical University named after I.I.  Mechnikov. Russia , 191015, Saint Petersburg, Kirochnaya Street, 41
2Almazov National Medical Research Centre, Russia, 197341, Saint Petersburg, Akkuratova Street, 2
Abstract
Introduction. Elderly age is a risk factor for unfavorable course and prognosis in acute coronary syndrome without ST segment elevation. Comorbidity is often founded in elderly patients masking the course of the underlying disease.
The aim of the study was to reveal the clinical features of acute coronary syndrome with non-ST segment elevation in patients of different age groups.
Materials and methods. It was compared the course of acute coronary syndrome in patients of 3 age groups during hospitalization and in 1 year of examinations.
Results. Comorbid conditions were mainly observed in elderly patients, atypical clinical picture of acute coronary syndrome was prevailed. Despite the high risk of hospital and 6-month mortality in elderly patients, these patients underwent percutaneous coronary interventions less frequently.
Conclusion. Acute coronary syndrome without ST segment elevation in elderly patients is characterized by atypical course and severe comorbid conditions resulted in more frequent complications and determines the choice of treatment plan.
Key words: coronary heart disease, acute coronary syndrome non-ST elevation, frailty, percutaneous coronary intervention, stenting.

STUDY OF THE IMMUNOTROPIC EFFECTS OF FLAVONE GLYCOSIDE EMBININ BY FLOW CYTOMETRY
N.V. Bychkova1.2, A. A. Kalashnikova1.2, A. K. Whaley3, V. G. Luzhanin3, N.M.Kalinina1.2, E. B. Shustov3, S. V. Okovity3
1Nikiforov Russian Center of Emergency and Radiation Medicine, Russia, 194044, Saint- Petersburg, Academica Lebedeva Street, 4/2
2 Pavlov First Saint Petersburg State Medical University, Russia, 197022, Saint- Petersburg, Lev Tolstoy Street, 6-8
3Saint-Petersburg State Chemical Pharmaceutical University, Russia, 197376, Saint -Petersburg, Professor Popov Street, 14A.
Abstract
Introduction. Currently, there is still a problem to find pharmacological substance with immunomodulatory properties.
 The aim of the study is to evaluate the embinin immunomodulatory effects on different populations of effectors cells of the immune system in vitro.
Materials and Methods. Flow cytometry was used to study the effect of embinin on the activation markers expression on the lymphocytes of healthy controls during the whole blood cultivation. The proliferative and cytotoxic activities of whole blood lymphocytes in the presence of a standard inductor and different doses of the test compound was studied.
 Results. It was found an increase in the expression of early activation marker CD69 on all studied cell populations and CD38 on T- and NKT-lymphocytes. It was revealed the effect of killing stimulation of target cells K-562 T- and NKT-cells. It was found the embinin inhibitory effect on the proliferative activity of lymphocytes.
Conclusion. Embinin has an immune stimulating effect.
Key words: embinin, flow cytometry, functional activity of cells of the immune system

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